https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24304 485) (P < 0.01), p-PDPK-1 (Ser⁴¹) (P < 0.05), and PKCζ (P < 0.05) was higher in the PCUN and PIUN groups, and hepatic PDK4 (P < 0.001) and CPT-1 (P < 0.05) protein abundance was also higher in the PIUN twin fetus. We also found that the expression of a number of microRNAs was altered in response to PCUN or PIUN and that there is evidence that these changes may underlie the changes in the protein abundance of key regulators of hepatic fatty acid β-oxidation in the PCUN and PIUN groups. Therefore, embryo number and the timing of maternal undernutrition in early pregnancy have a differential impact on hepatic microRNA expression and on the factors that regulate hepatic fatty acid oxidation and lipid synthesis.]]> Wed 28 Feb 2024 15:22:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28269 Wed 11 Apr 2018 15:33:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27351 Wed 11 Apr 2018 13:32:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16992 Wed 11 Apr 2018 11:11:09 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26912 Wed 11 Apr 2018 10:53:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35270 Wed 06 Apr 2022 14:02:10 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29979 Wed 04 Sep 2019 10:18:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33387 34-weeks gestation; n=8) and gestational age matched preterm (31.6-35.1 weeks; n=8) and term normotensive controls were also compared. Agilent Human miRNA microarray v19 was used to detect up to 2006 miRNAs in four placentae from each group. Statistically different levels of expression were determined and refined using predictive modelling. Placental miRNAs predicted to target RAS mRNAs were identified in three databases. Differences detected on the array were confirmed for some miRNAs by semi-quantitative RT-PCR (qPCR, n=7-8 for all groups). Two differentially expressed miRNAs that were known to target human renal REN mRNA (miR-181a-5p and miR-663) were transfected into human HTR-8/SVneo trophoblast cells to examine their effect on placental REN expression and prorenin levels. In early gestation placentae, 186 miRNAs were differentially expressed compared with term placentae (109 increased, 77 decreased). Thirty of the differentially expressed miRNAs were predicted to target RAS components. In mid-gestation placentae, 117 miRNAs were differentially expressed compared with term placentae (69 increased, 48 decreased). Of these, 19 had RAS mRNAs as predicted targets. Eight miRNAs that were lower in early gestation and predicted to target RAS mRNAs were confirmed by qPCR. All showed an increase during gestation and could influence the transgestational profile of the human placental RAS. Additionally, on the array, three miRNAs predicted to target RAS mRNAs (miR-892c-3p, miR-378c and miR-514b-3p) were overexpressed in placentae from women with late-onset PE (P = 3.6E-10, P = 1.8E-05, P = 5.3E-06; respectively). miR-663, which suppresses renal REN mRNA expression, was overexpressed in early-onset PE placentae as determined by qRT-PCR analysis (P = 0.014). Transfection of miR-181a-5p and miR-663 into HTR-8/SVneo trophoblast cells suppressed REN mRNA expression (p = 0.05) and prorenin protein production (P = 0.001). Data can be found via GEO accession number GSE109832. Further validation that the differentially expressed miRNAs do indeed directly target RAS mRNAs and affect placental development and function is required. This study is limited by the small sample size. Therefore independent validation in a larger cohort is required.]]> Wed 02 Mar 2022 14:28:37 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19185 Tue 24 Aug 2021 14:25:09 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20824 Sat 24 Mar 2018 08:05:56 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16908 Sat 24 Mar 2018 07:59:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20508 Sat 24 Mar 2018 07:59:05 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20984 Sat 24 Mar 2018 07:50:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19600 ACE A11860G genotype is associated with small for gestational age babies (SGA) and to determine whether the association is affected by environmental factors and fetal sex. Overall, 3234 healthy nulliparous women with singleton pregnancies, their partners and babies were prospectively recruited in Adelaide, Australia and Auckland, New Zealand. Data analyses were confined to 2121 Caucasian parent–infant trios, among which 216 were pregnancies with SGA infants and 1185 were uncomplicated pregnancies. Women with the ACE A11860G GG genotype in the combined and Adelaide cohorts had increased risk for SGA [odds ratios (OR) 1.5, 95% confidence interval (CI) 1.1–2.1 and OR 2.0, 95% CI 1.3–3.3, respectively) and delivered lighter babies (P = 0.02; P = 0.007, respectively) compared with those with AA/AG genotypes. The maternal ACE A11860G GG genotype was associated with higher maternal plasma ACE concentration at 15 weeks' gestation than AA/AG genotypes (P < 0.001). When the Adelaide cohort was stratified by maternal socio-economic index (SEI) and pre-pregnancy green leafy vegetable intake, the ACE A11860G GG genotype was only associated with an increased risk for SGA (OR 4.9, 95% CI 1.8–13.4 and OR 3.3, 95% CI 1.6–7.0, respectively) and a reduction in customized birthweight centile (P = 0.006 and P = 0.03) if superimposed on maternal SEI <34 or pre-pregnancy green leafy vegetable intake <1 serve/day. Furthermore, the associations of maternal ACE A11860G with customized birthweight centile observed among Adelaide women with SEI <34 or pre-pregnancy green leafy vegetable intake <1 serve/day were female specific. The current study identified a novel association of maternal ACE A11860G with SGA. More interestingly, this association was modified by environmental factors and fetal sex, suggesting ACE A11860G–environment–fetal sex interactions.]]> Fri 17 Nov 2023 11:54:01 AEDT ]]>